The C. elegans ORFeome cloning project
| Sequencing information (OST): | |
| OST in ORFeome version 1 confirms ORF (WS9) but cloned out of frame | |
| OST in ORFeome version 3 does not confirm repredicted ORF (WS100) | |
| See below for graphical display | |
| ORFeome 3 | |
| ID (wormbase WS100): R02F2.4 | size: 1296 |
| Original Position: 30010@A12 | chromosome: III |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttgagattattgccttgtctaacg Reverse primer: ggggaccactttgtacaagaaagctgggtattggcattgctctagaagtg Predicted Sequence: atgagattattgccttgtctaacgactctacttctcacgatatcaactgtaaactcgaaatttgtcacagattgcacgaa tgttggagacggaatgtaccctctgggtgcctgtgaaccgagattcctagcttgcgtcagcggcgaggctcgatatatgg actgcccggaggatctggtttatcacaaaaatctggagttttgtgattggaggcacaatgtgtttgaatgtggagaggaa ggagaagaaaacgagttttctggtgatggttccggtgaaagctcaggagacgaagaaatcacatttggcgattcgtcggg agaatcttctggcgacgagctgctagaaaatgtgtgcgagagtctcaaagatggcgtctactcctccggcacttgcagta gctcctatattatttgcaactccggctcacctcgattcctcagctgctcaacacctcttatctacgatccaaccaataaa aagtgttcatggaaaggaatgattgatgaatgttctcaagtatcaggagaatattgtgaatccgatggaaatatctcaaa gtccgagtgctccaatgttttcttctcgtgttctgaaggcatcgctcaccgtagaaattgcccggcgaatcttgtcttca atccagcgatttcctcttgtgattggccaaaaaatgtgatggattgttctgaaaaatctgaaaagccacaaaactgcggc gaagtcgacggatatttctcatttggcagatgctcttcatcattttcagcctgtacaaatggcattccaattgtcatgtt ttgtccagatgggctaatgttttccgagaaaaatcaaatgtgtgactatgaatggaacgttgacgagtgtgacttggaat catctggatttatggaaaactacaaagccagtgaagccttaacgccctgcacaaatatggacaatggtctctatgctctc gattgtaccccgcgagttctatcgtgtcaaaatggacgagagaatatttttgagtgcccaccgagtctggttttcaatga gaattctttgatatgtgactacccggagacttctctgaaatgctgcatggaagacgcattgctcattcgtgatgcctcca ttggaacatacgactgctccatcgacgggctcttctcaagtactttatgctcccgtaactaccacaaatgctccaacggc cagctcatcccacacgaatgtgctgattcgaatgctgttttctctgcaagtgctgccaaatgcgtggattcttcacttct agagcaatgccaataa | |
| ORFeome 1 | |
| ID (wormbase WS100): R02F2.4 | size: 1290 |
| In Frame version 1: N | chromosome: III |
| Original Position: 10057@E8 | Clone available in well 11301@D12 (see OST) |
| ORF is available as RNAi clone in GHR-11301@D12 | |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttgttcttcaagcaaaatgagattattg Reverse primer: ggggaccactttgtacaagaaagctgggtattggcattgctctagaagtg Predicted Sequence: atgttcttcaagcaaaatgagattattgccttgtctaacgactctacttctcacgatatcaactattgcacgaatgttgg agacggaatgtaccctctgggtgcctgtgaaccgagattcctagcttgcgtcagcggcgaggctcgatatatggactgcc cggaggatctggtttatcacaaaaatctggagttttgtgattggaggcacaatgtgtttgaatgtggagaggaaggagaa gaaaacgagttttctggtgatggttccggtgaaagctcaggagacgaagaaatcacatttggcgattcgtcgggagaatc ttctggcgacgagctgctagaaaatgtgtgcgagagtctcaaagatggcgtctactcctccggcacttgcagtagctcct atattatttgcaactccggctcacctcgattcctcagctgctcaacacctcttatctacgatccaaccaataaaaagtgt tcatggaaaggaatgattgatgaatgttctcaagtatcaggagaatattgtgaatccgatggaaatatctcaaagtccga gtgctccaatgttttcttctcgtgttctgaaggcatcgctcaccgtagaaattgcccggcgaatcttgtcttcaatccag cgatttcctcttgtgattggccaaaaaatgtgatggattgttctgaaaaatctgaaaagccacaaaactgcggcgaagtc gacggatatttctcatttggcagatgctcttcatcattttcagcctgtacaaatggcattccaattgtcatgttttgtcc agatgggctaatgttttccgagaaaaatcaaatgtgtgactatgaatggaacgttgacgagtgtgacttggaatcatctg gatttatggaaaactacaaagccagtgaagccttaacgccctgcacaaatatggacaatggtctctatgctctcgattgt accccgcgagttctatcgtgtcaaaatggacgagagaatatttttgagtgcccaccgagtctggttttcaatgagaattc tttgatatgtgactacccggagacttctctgaaatgctgcatggaagacgcattgctcattcgtgatgcctccattggaa catacgactgctccatcgacgggctcttctcaagtactttatgctcccgtaactaccacaaatgctccaacggccagctc atcccacacgaatgtgctgattcgaatgctgttttctctgcaagtgctgccaaatgcgtggattcttcacttctagagca atgccaataa | |
