The C. elegans ORFeome cloning project
| Sequencing information (OST): | |
| OST in ORFeome version 1 does not confirm ORF (WS9) | |
| OST in ORFeome version 3 confirms ORF (WS100) | |
| See below for graphical display | |
| ORFeome 3 | |
| ID (wormbase WS100): H18N23.2a | size: 1041 |
| In Frame version 3: Y | chromosome: X |
| Original Position: 30029@G03 | Clone available in well 31013@B02 at Open Biosystems (see OST) |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttggaaatggggcgcgaacgagaa Reverse primer: ggggaccactttgtacaagaaagctgggtaccagtacggaccatcggtgcta Predicted Sequence: atggaaatggggcgcgaacgagaatgctgcgtcctctccacatcgccgggctgccgccgagccgcaagcttcccctttgt cggcgactcctcaataatacaggcatccagtttgaaggtccgctccaagagcgagtcggaagccttaaacaagatgcgat tgacacagatgaacttggaaacggttcgcaaacgtctgtccgaatcgacaattaaggagaaagttccagatgaaggaagc gactaccaatcgcacagttcatcgagcagcgaatgcgaggtcgacgatttggagctcttctgtgcggggcctctcgaatt gaaagaggaagagaagttgcgcaaatcggtacgatttgccgacgactgcggacatgagctctacatggtccgcgtggcga cggagcccagcaattgcccaccgaagctgagtccttctgtgctccgtcgctatcggggagaatcgttcgaagaagaggag atgactactcgcgagccggctcctgtctggaatctgatgtttaaacagcctgcagcggattatgttcgttttcgggaaaa catggaaactaacaaagtgtcgctggaaaatgtgatcttaaacaatgaagcatacaaagtgatgggaacaatcaaagtgg gcaatattgcatttgagaagagcgtttttgtgcgatacacaatgaatggctggatttcgtatatggataaagcggcgata taccaaccatccaccagcaaaattcaggacactttcaaattcgaccttgacctgccctcttctgtggagaagatccacca aattgagttttgcatttgtttcaaagcgaacggcaccgaatactgggactctaactcgggtacaaactacgtgttgcact gcgagcagcaacagcatcctgccgttccacaacaacgtcgcgcgtcgttgttcggcagttcaaaatatctcgaccgagat gacgcctacaagctcgactacaacgattggggaaaattcgcctcttggaaggcgcttagcaccgatggtccgtactggta g | |
| ORFeome 1 | |
| ID (wormbase): H18N23.2a ID (WS9): F15G10.1 | size: 897 |
| Original Position: 10209@G7 | chromosome: X |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttgcgtcagcctacgaatgaa Reverse primer: ggggaccactttgtacaagaaagctgggtacctgccgaacaacgacg Predicted Sequence: atgcgtcagcctacgaatgaaggaagcgactaccaatcgcacagttcatcgagcagcgaatgcgaggtcgacgatttgga gctcttctgtgcggggcctctcgaattgaaagaggaagagaagttgcgcaaatcggtacgatttgccgacgactgcggac atgagctctacatggtccgcgtggcgacggagcccagcaattgcccaccgaagctgagtccttctgtgctccgtcgctat cggggagaatcgttcgaagaagaggagatgactactcgcgagccggctcctgtctggaatctgatgtttaaacagcctgc agcggattatgttcgttttcgggaaaacatggaaactacaatcattgatcttaatgctgaatacactggtgccaaaacat ttgttatatctgaaatgttaattttctttgatttgtataaaccttttgactcaaaactttttaataaaccacttatatat tatttttacttatattcaaacttgaatttttccttacagaacaaagtgtcgctggaaaatgtgatcttaaacaatgaagc atacaaagtgatgggaacaatcaaagtgggcaatattgcatttgagaagagcgtttttgtgcgatacacaatgaatggct ggatttcgtatatggataaagcggcgatataccaaccatccaccagcaaaattcaggacactttcaaattcgaccttgac ctgccctcttctgtggagaagttacagatccaccaaattgagttttgcatttgtttcaaagcgaacggcaccgaatactg ggactctaactcgggtacaaactacgtgttgcactgcgagcagcaacagcatcctgccgttccacaacaacgtcgcgcgt cgttgttcggcaggtga | |
| ORFeome 1 | |
| ID (wormbase WS100): H18N23.2a ID (WS9): F15G10.2 | size: 399 |
| In Frame version 1: Y | chromosome: X |
| Original Position: 10203@G3 | Clone available in well 11073@G06 at Open Biosystems and MRC geneservice (see OST) |
| ORF is available as RNAi clone in GHR-11073@G06 | |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttgggcttctcactttccat Reverse primer: ggggaccactttgtacaagaaagctgggtaattgtcgattcggacagacgtt Predicted Sequence: atgggcttctcactttccatgctcatcgacagttttgaagatttagacggtgtgattcaggagatcaagtttcgattgac taggttcgtttgcagtgggcccccctctccgactattgtttgttattgtaaggttttctgctttatacttttctatttca aagcattgctgctcctaggcgccgatggaaatggggcgcgaacgagaatgctgcgtcctctccacatcgccgggctgccg ccgagccgcaagcttcccctttgtcggcgactcctcaataatacaggcatccagtttgaaggtccgctccaagagcgagt cggaagccttaaacaagatgcgattgacacagatgaacttggaaacggttcgcaaacgtctgtccgaatcgacaattaa | |
| ORFeome 1 | |
| ID (wormbase WS100): H18N23.2a ID (WS9): H18N23.a | size: 1191 |
| In Frame version 1: Y | chromosome: X |
| Original Position: 10003@F3 | Clone available in well 11085@G07 at Open Biosystems and MRC geneservice (see OST) |
| ORF is available as RNAi clone in GHR-10003@F3 | |
Forward primer: ggggacaagtttgtacaaaaaagcaggcttggggcgcgaacgagaat Reverse primer: ggggaccactttgtacaagaaagctgggtgccagtacggaccatcggtgctaa Predicted Sequence: atggggcgcgaacgagaatgctgcgtcctctccacatcgccgggctgccgccgagccgcaagcttcccctttgtcggcga ctcctcaataatacaggcatccagtttgaaggtccgctccaagagcgagtcggaagccttaaacaagatgcgattgacac agatgaacttggaaacggttcgcaaacgtctgtccgaatcgacaattaaggagaaagatgaaggaagcgactaccaatcg cacagttcatcgagcagcgaatgcgaggtcgacgatttggagctcttctgtgcggggcctctcgaattgaaagaggaaga gaagttgcgcaaatcggtacgatttgccgacgactgcggacatgagctctacatggtccgcgtggcgacggagcccagca attgcccaccgaagctgagtccttctgtgctccgtcgctatcggggagaatcgttcgaagaagaggagatgactactcgc gagccggctcctgtctggaatctgatgtttaaacagcctgcagcggattatgttcgttttcgggaaaacatggaaactac aatcattgatcttaatgctgaatacactggtgccaaaacatttgttatatctgaaatgttaattttctttgatttgtata aaccttttgactcaaaactttttaataaaccacttatatattatttttacttatattcaaacttgaatttttccttacag aacaaagtgtcgctggaaaatgtgatcttaaacaatgaagcatacaaagtgatgggaacaatcaaagtgggcaatattgc atttgagaagagcgtttttgtgcgatacacaatgaatggctggatttcgtatatggataaagcggcgatataccaaccat ccaccagcaaaattcaggacactttcaaattcgaccttgacctgccctcttctgtggagaagatccaccaaattgagttt tgcatttgtttcaaagcgaacggcaccgaatactgggactctaactcgggtacaaactacgtgttgcactgcgagcagca acagcatcctgccgttccacaacaacgtcgcgcgtcgttgttcggcagttcaaaatatctcgaccgagatgacgcctaca agctcgactacaacgattggggaaaattcgcctcttggaaggcgcttagcaccgatggtccgtactggtag | |
